超级坏蛋格鲁（史蒂夫·卡瑞尔 Steve Carell 配音）在听说埃及金字塔被盗的新闻后不满市面上新贼辈出，决定在纳法利欧博士（拉塞尔·布兰德 Russell Brand 配音）的帮助下建造火箭升空盗取月亮。在向坏蛋银行贷款的过程中，格鲁盗取的收缩射线枪被新贼维克托（杰森·席格尔 Jason Segel 配音）抢走。为了夺回收缩射线枪，格鲁决定领养三位孤儿——玛戈（米兰达·卡斯格拉夫 Miranda Cosgrove 配音）、伊迪丝（达娜·盖伊 Dana Gaier 配音）和阿格蕾丝（埃尔希·费舍 Elsie Fisher 配音），利用她们进入维克托的城堡兜售饼干的机会实施偷盗，计划成功后再偷偷处理掉三个小孩。然而在平凡的日常相处中，格鲁却发现自己越来越离不开这三个小鬼，但这样的感情用事却将严重影响周密的盗月计划……
Dr. Nefario：Thank you!
Gru：There he is. He's styling. Now, we have located a shrink ray in a secret lab, and once we take this shrink ray, we will have the capability to pull off the true crime of the century. We are going to steal... Wait, wait! I haven't told you what it is yet. Hey. Dave, listen up, please. Next, we are going to steal, pause for effect, the moon! And once the moon is mine, the world will give me whatever I want to get it back! And I will be the greatest villain of all time! That's what I'm talking about. Yes?
Dr. Nefario：Hello, Gru? I've been crunching some numbers, and I really don't see how we can afford this. It can't be done. I'm not a miracle worker.
Gru：Hey, chillax. I'll just get another loan from the bank. They love me!