Meghan Markle’s estranged half-sister strikes again.

Samantha Markle is accusing the American actress-turned-Duchess of Sussex of having “no heart” in a slamming new interview.

The 54-year-old participated in a new documentary titled “Meghan and the Markles: A Family at War,” The Daily Mail reported Friday.

The special, which is airing in the U.K., aims to look at the reasons why none of Markle’s relatives, apart from her mother Doria Ragland, were present in the widely televised wedding last year.

It also highlights an interview with Princess Diana’s biographer Andrew Morton, who discusses how other relatives have “heaped embarrassment upon her.”

In the tell-all, the Mail reported, Samantha discussed the letter Markle, 37, sent to their father, former Hollywood lighting director Thomas Markle, shortly after she married Britain’s Prince Harry in May 2018.

In the emotional letter, which Thomas, 74, gave the UK-based publication permission to publish in February of this year, Markle revealed the patriarch broke her heart “into a million pieces” by speaking out to the press without her consent, fabricating stories and attacking her new husband.

Samantha slammed the letter, claiming Markle refused to help her father cope with the intense media scrutiny following her engagement to Harry in late 2017.

Thomas told The Daily Mail he originally planned to keep the letter “totally private out of respect for her” until her friends “launched their attack” on him in a shocking People magazine interview where they spoke candidly about the family rift.